Dilution Calculator (C1V1 = C2V2)
Use this tool to calculate how much stock solution and diluent you need to make a target percent solution.
A percent solution dilution calculator helps you quickly determine how much concentrated stock solution to mix with a diluent (usually water) to reach a desired concentration. This is one of the most common calculations in chemistry labs, classrooms, microbiology prep, and even household cleaning tasks.
How the calculator works
The core relationship is:
C1V1 = C2V2
- C1 = starting concentration (stock)
- V1 = volume of stock you need to use
- C2 = final desired concentration
- V2 = final total volume after dilution
Once V1 is found, the diluent volume is simply V2 − V1.
Step-by-step example
Problem
You have a 25% stock solution and want 1,000 mL of a 4% solution.
Solution
- Use the equation: V1 = (C2 × V2) / C1
- V1 = (4 × 1000) / 25 = 160 mL stock
- Diluent = 1000 − 160 = 840 mL
So you would mix 160 mL stock + 840 mL diluent.
Important notes about percent solutions
1) Know what “percent” means in your context
- % w/v: grams solute per 100 mL solution
- % v/v: mL solute per 100 mL solution
- % w/w: grams solute per 100 g solution
Use the same basis for both stock and target concentrations. Mixing bases will produce incorrect results.
2) Unit consistency matters
Volumes can be mL, L, µL, or other units, but all volume terms must use the same unit. The calculator assumes your input and output share that chosen unit.
3) Dilution only lowers concentration
If your target concentration is greater than the stock concentration, you cannot reach it by dilution alone. You would need a more concentrated stock or evaporation/concentration steps.
Where this calculator is useful
- Preparing disinfectant and sanitizer working solutions
- Making buffer solutions in biology and chemistry labs
- Serial dilutions for microbiology and analytical testing
- Food and beverage formulation adjustments
- Educational lab exercises and exam practice
Common mistakes to avoid
- Entering concentration values as decimals instead of percentages (e.g., use 5, not 0.05)
- Confusing final volume with volume of diluent
- Using mismatched concentration definitions (% w/v vs % v/v)
- Rounding too early in multi-step procedures
Quick FAQ
Can I use this for serial dilutions?
Yes. Run each step separately with the new intermediate concentration as the next stock concentration.
What if the target concentration is 0%?
That means no stock solution is needed; your final volume is entirely diluent.
Do I need to adjust for temperature?
For most everyday and teaching uses, no. For high-precision analytical work, temperature and density corrections may be necessary.